From EC terms to GO terms to GO enrichment
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5 months ago

Hi

I did a KEGG annotation of RNAseq data. I want do do GO enrichment next.

I do have the EC numbers of the genes of interest, so I converted the EC numbers to GO terms using EC2GO. But I only get one GO per EC term. Can I perform GO enrichment analyses using only a single GO term? Ive seen that some enzymes/proteins have multiple GO terms.

Or what should I do? Any help would greatly appreciated.

Thanks

enrichment EC terms GO • 594 views
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Is there any specific reason for doing GO enrichment using EC numbers? I mean why don't you perform GO enrichment analysis using gene symbols obtained by RNA-Seq analysis?

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We only got the full Accession number, full gene names (we do not have gene symbols) and associated EC terms from the KEGG annotation.

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What accession numbers do you have. You can obtain the gene symbol using accession. For example, you can use biomaRt for ids conversion.

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Unfortunately biomart does not contain KEGG identifiers

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We only got the full Accession number

You said you have accession numbers, so which accession numbers do you have?

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e.g. K11187

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Please use ADD REPLY when replying to existing comments.

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OK will do