GSEA and over-representation analysis of many genes
0
0
Entering edit mode
3.3 years ago
avelarbio46 ▴ 30

Hello everyone! I've been doing some Differential Expression analysis on specific samples. It happens that I found a lot of genes that are DE. In total, of 24000 features, 11000 were up or down regulated in control vs group. Even tough the number is very high, I've very good reasons and evidences to believe these numbers.

My question is: how well ORA and GSEA algorithms handle large numbers of genes? I tried using cluster profiler with wikipathways, reactome, cell type and KEGG, but I only got results with p-values below 0.05 for KEGG with ORA analysis.

But, seing that half the genes are DE, I expected way more significative results, which made me question how well those methodologies handle very large number of genes!

Can anyone illuminate me?

GSEA DE ORA RNAseq • 865 views
ADD COMMENT
0
Entering edit mode

How will you draw the plot for ORA and GSEA?? I also identified DEG for two different dataset and find common DEG from them.But for that two dataset I got two adj.p.value.because of two dataset.So now how can i generate the plot???

ADD REPLY

Login before adding your answer.

Traffic: 2371 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6