regarding gene expression data of GDAC Firehouse database

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2.7 years ago

jyotsnap • 0

Hello guys, can i use broad gdac firehouse database normalized gene expression data directly without any preprocessing...its mRnaseq (illuminahiseq_rnaseqv2-RSEM_genes_normalized (MD5)) data, obtained from database.

analysis data Rnaseq • 766 views

ADD COMMENT • link 2.7 years ago by jyotsnap • 0

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I think yes, why not? But what sort of analysis you are going to perform with this dataset? I mean is it comparative (normal Vs tumor, Tumor Grade etc.) OR survival?

ADD REPLY • link 2.7 years ago by Nitin Narwade ★ 1.6k

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Thanks for reply...I am doing both differential expression analysis and survival analysis by taking these data from Broad GDAC Firehouse database, only confusion is whether i have to use them directly or any processing required, expression data file header already showing "normalized count " which means it is alredy normalized..i guess

BLCA_Rnaseq_expression_clinical_data

ADD REPLY • link 2.7 years ago by jyotsnap • 0

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