can i use broad gdac firehouse database normalized gene expression data directly without any preprocessing...its mRnaseq (illuminahiseq_rnaseqv2-RSEM_genes_normalized (MD5)) data, obtained from database.
I think yes, why not?
But what sort of analysis you are going to perform with this dataset? I mean is it comparative (normal Vs tumor, Tumor Grade etc.) OR survival?
Thanks for reply...I am doing both differential expression analysis and survival analysis by taking these data from Broad GDAC Firehouse database, only confusion is whether i have to use them directly or any processing required, expression data file header already showing "normalized count " which means it is alredy normalized..i guess
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