How to adjust sequence line to 60bp?

Question

[mthm]

I have a rather primitive question

After importing my sequence alignments to either "Geneious" or "BioEdit", I cannot export them in the same 60pb per line format, does anyone know how to do that to avoid the manual line adjusting? Alternatively if there is a web-based line width convertor (e.g. 80 to 60 or 100 to 60 or infinite to 60,..) to solve the problem quickly, please let me know

Answer 1

[Jorge Amigo]

The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing. You may install them and use them locally.

Alternatively, its ability to modify FASTA width is available on Galaxy under the name "FASTA Width formatter".

Answer 2

[colindaven]

This one might do it, not sure:

https://www.ebi.ac.uk/Tools/sfc/emboss_seqret/

or in Galaxy there is a tool called: FASTA Width formatter

Answer 3

[lieven.sterck]

unix command fold can format lines on defined length

Answer 4

[GenoMax]

reformat.sh from BBMap suite. Set NN to desired number.

reformat.sh -Xmx2g in=your.fa out=reformat.fa fastawrap=NN