I'm using a software that requires TPM. But I can't find enough RNAseq data to do analysis at the moment. I have downloaded some microarray data from a database that meets the analysis requirements. The original data were obtained using Affymetrix Human Genome U133A 2.0 Array.
Since I have not done microarray-related analysis before, I don't know about the relationship between microarray data and RNAseq data.
My question is that whether the probe intensity of microarray can be used to calculate TPM like the count data of RNAseq?
Thank you in advance for your answers!
Hello, I have the same problem as you. Would it make sense to multiply the intensity values of the probes by a sufficiently large number (e.g. x 10000) and round up to get a sort of "count". I have no idea if this is possible or not...
No, that would not make sense at all.
What analysis are you trying to do that requires TPMs?