Using a large public single-cell RNA-seq dataset from brain where cells are already segregated by brain region, cell type, marker gene cluster, etc. I am looking to do exploratory analyses to learn whatever I can about a candidate gene of interest. What kinds of strategies are recommended? Here are some I thought of already.
Run co-expression analysis (WGCNA) on clusters or subclasses (25); identify modules/hubs that include the gene of interest; run Gene Ontology enrichment analyses, compare ontologies across clusters/subclasses
Identify the marker genes of each cluster using Seurat; can also find conserved markers across all clusters/subclasses
Gene regulatory network reconstruction; Identify regulons containing the gene of interest, figures with iRegulon or Cytoscape
Trajectory analysis - how does the gene of interest's expression change over cell regulatory/developmental trajectories?
Any ideas are appreciated!