What is the best program or way to solve undefined protein subcellular localizations?

Question

[BATMAN]

Hi, I'm analyzing the subcellular localization of the proteins of a constructed pangenoma, using Cello 2.5 and PSORTB.I wanted to ask for some advice to further refine the output of each program and reach a consensus since I'm analyzing a pangenome with 4800 proteins with PSORTB and I get 1900 proteins whose subcellular localization is undefined. Is there any other program to analyze this? Any advice?

Answer 1

[Mensur Dlakic]

Several methods I have tried before:

• http://busca.biocomp.unibo.it/
• http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/
• http://www.cbs.dtu.dk/services/SignalP/
• https://rostlab.org/services/loctree3/

A long list:

https://www.ncbi.nlm.nih.gov/CBBresearch/Lu/subcellular/

[BATMAN]

thanks a lot!