Deleted:How much starting library to use for Ion Torrent S5 sequencing?
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2.1 years ago

For Ion Torrent S5 sequencing, the user guide for IonChef/S5 recommends 25-60pM of library for each chip, depending on chip type and library fragment size.

Ref: (Page 26)

I was just told to start with 30pM of library for 530chip, and then adjust from there by looking at the quality of reads and polyclonality. I am wondering: is there a way to properly calculate or estimate the amount of library (in pM) to load onto a chip?

loading concentration chip library ion starting torrent sequencing • 338 views
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