bcl2fastq ERROR

Question

[GiV17]

Hi all, I have a problem. I'm running bcl2fastq software on Ubuntu, but I can't demultiplex anything as the software gives me this error:

 *ERROR: bcl2fastq::common::Exception: 2021-Sep-02 12:12:34: Success (0): /home/Software/bcl2fastq/src/cxx/lib/layout/Layout.cpp(693): Throw in function void bcl2fastq::layout::handleDefaultSample(bool, bool, size_t, bcl2fastq::layout::LaneInfo&, bool&)
Dynamic exception type: boost::exception_detail::clone_impl<bcl2fastq::common::InputDataError>
std::exception::what: Missing a barcode in lane 1*

I don't Understand why... help me. thanks

[colindaven]

please paste your samplesheet

Answer 1

[GenoMax]

Missing a barcode in lane 1

Check your SampleSheet file to make sure that gets addressed.

Answer 2

[GiV17]

Thanks all, I checked the Sample-Sheet and actually there were 3 commas (,) too many that did not allow the correct reading.