Gene with hundreds of counts very similar, is it possible to use it to get DEGs?
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12 months ago
Anderson • 0

Hi, I'm starting in boinfomatics and I'm using Deseq2 for differential expression analysis. I have an RNAseq dataset, where one of the genes I intend to analyze has hundreds of counts ranging from 10 to 12, with a few counts > 9000 (not identified as outliers by the program). I process this data in Deseq2 and get that the gene is differentially expressed across several samples of interest. What can justify these strange counts? Are the results reliable?

DEG Deseq2 similar couts • 443 views
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here one of the genes I intend to analyze has hundreds of counts ranging from 10 to 12, with a few counts > 9000

Can you confirm that you are referring to a single gene that has variable counts across many samples?

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That's exactly it

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