I'm very new to single cell clustering, but have been able to get results from Seurat using sample datasets from 10x Genomics and also some datasets that were in H5 format.
I'm looking for scRNA-seq datasets that are specific to traumatic brain injury (TBI). So far I haven't found any datasets that are in a format I am familiar with. One looks promising: GSE101901. I'm not sure how to load this one into Seurat... there are several other GEO TBI-related datasets out there but it's the same problem.
Can you show me how to either 1) find a TBI-related scRNA-seq dataset in a format I am familiar with or 2) successfully create a seurat object with the GSE101901 dataset?
Thank you for your help, I've been stuck on this for some time!
Thank you so much!
I used the similar method for a few GEO databases and they worked very well. However I got something very weird from the following GEO database: https://ftp.ncbi.nlm.nih.gov/geo/samples/GSM5555nnn/GSM5555214/suppl/GSM5555214_Vthunter_0029_count_new_data_20210531.txt.gz