Reads preparation steps for amplicon metagenomics
0
0
Entering edit mode
2.6 years ago
Denis ▴ 310

Hi,

I'm analysing amplicon data for an metagenomics study. I'm wondering if i have to use only read pairs containing primer sequences on the 5' end allowing some proportion of the mismatches in them of course? I think this step could help to remove PCR artifacts from the real biological data. Is it a useful approach for amplicon metagenomics?

PCR Metagenomics • 448 views
ADD COMMENT

Login before adding your answer.

Traffic: 2133 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6