Novel Splice Variant Discovery from RNAseq Data
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3.1 years ago
saipra003 ▴ 10

Hi Everyone! I'm fairly new to the field of bioinformatics. I recently received cancer RNAseq data from my PI who asked me to find "novel" splice variants in the data so we can explore them further.

Does anyone have any idea on how to accomplish this?

I've fed the data through some pipelines like rMATS and SUPPA but both of them seem to detect differential splice variant expression based on a reference annotation file instead of detecting novel splice variants.

Variants Splice Computational RNAseq • 534 views
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