Genome Sequencing - what is the 'identifier'?
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5 days ago
pollyyhjo • 0

What is the 'identifier' of the first read in both files? Here is the code I get. Also, what does this identifier of both reads tell us? enter image description here

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coding sequence identifier • 135 views
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Specifically: https://en.wikipedia.org/wiki/FASTQ_format#Illumina_sequence_identifiers

So there is no identifier (as far as a sample ID goes) inside an Illumina file. You would normally have that information in the name of the file. If someone "coded" the names to be generic (like what you have) then you had better have a key/metadata file that links the index sequence you see in header (GGACTCCT+CTCCTTAC) with a sample_ID/file names.

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So we cannot tell what identifier from the code above?

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Identifier for? If for sample, then no.

But if you wanted to know what sequencer the sample ran on then you get the serial number NB551191. Flow cell serial number is HM5WHBGX5. Data is from lane 1.

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