I'm dealing with
I'd like to apply
BBDuk to remove primers from the
5'end of my forward and reverse reads, but have a couple of questions. Could you help me please?
How may i indicate in command line which primer i need to clip from forward and which from the reverse reads?
There is the
BBDukparameter to restrict search within the leftmost bases of the read instead of the whole read sequence. Is it possible to set up this parameter separately for forward and reverse reads?