get read length from sra
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2.6 years ago
Nobody ▴ 30

Hi everyone!

I need a method "api" to retrieve reads length from SRA.

Thanks in advance

SRA rna-seq • 1.4k views
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You will only get average length of reads (which may or may not be very useful) using Entrezdirect.

$ esearch -db sra -query "SRR13310323" | efetch -format runinfo | cut -d ',' -f 1,7
Run,avgLength
SRR13310323,109
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The suggested methods works. Here is another method using the SRA toolkit

fastq-dump -X 1 -Z SRR5790106 | sed -n '2p' | awk '{ print length }'

Read 1 spots for SRR5790106

Written 1 spots for SRR5790106

100

this assumes that read length is the same for all reads

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