I just downloaded the blast command line applications and I would like to make a database of a MGEs nucleutides that i downloaded from ACLAME database site in fasta file. It is labeled as mydb.fasta and I have put the file into a bin folder of blast in the path C:\Program Files\NCBI\blast-2.12.0+\bin
Ive been typing this into the command line:
cd C:\Program Files\NCBI\blast-2.12.0+\bin to enter in directory
makeblastdb -in mydb.fasta -dbtype nucl -out DB
Building a new DB, current time: 09/23/2021 13:48:15 New DB name: C:\Program Files\NCBI\blast-2.12.0+\bin\DB New DB title: mydb.fasta Sequence type: Nucleotide Keep MBits: T Maximum file size: 1000000000B BLAST options error: File mydb.fasta does not exist
every time i got this error file does not exist. It is probably a simple problem to solve, but I cannot seem to get past it.