I find the sam PDF,and want to understand flag 0x2,but I can not understand.
where have some detailed instructions for it ?
in a paired end, both fragments are mapped on the same contig, anti-parallel to the other, at a good distance.
see slide 33 of https://www.slideshare.net/lindenb/next-generation-sequencing-file-formats-2017
Thanks a million.
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