Nonsense mutation and translation

Question

[vpsev3]

Does a nonsense mutation at the end of an exon prevent future exons of the gene from being translated into protein?

Answer 1

[Mensur Dlakic]

Any nonsense mutation stops the translation. However, you don't necessarily know the correct translation frame from a single exon, so it may not mean anything.

[vpsev3]

So if there is no other start codon in other exons, the translation stops and the other exons will not be translated into protein?

[Mensur Dlakic]

You are asking me to make a definitive statement without providing much information. The answer is that I don't know. That is because I don't know whether your exon is first, last, or in the middle. Also because it is almost impossible for any exon of decent size not to have a start codon. Or maybe you don't have the first exon. There are all kinds of answers to your question and you will most likely have to figure it out on your own after some investigation.

[vpsev3]

I see, the exon is in the middle

Is there a tool to check by entering the gene sequence?

[Mensur Dlakic]

You can always try to concatenate all the exons in order, and predict the ORF from that combined sequence. Chances are very high that a nonsense codon at the end of a middle exon (or really anywhere in the middle exon) is nothing to worry about.

I think you need to reconsider how you ask the questions. If you provided all the information in the beginning, it could have been answered with a single post. Instead, you are asking single-sentence questions, and we are going back and forth. And if some of my comments have provided clarity on your question, it is customary to upvote and/or accept the answer(s).