How to show relabel sub cluster on the original cluster in Seurat

Question

[bt20022]

Hi all!

I have some data that I've been clustering using Seurat. I ended up with 30 clusters, but I subset 3 of these clusters are reclustered them in order to get more specific cell types. I now have 7 clusters in this subset, but want to relabel the cells in the original object with the 30 clusters. Is this possible? Would really appreciate some help!

Answer 1

[Pratik]

Generally you should be able to use SetIdent().

I wrote a concise tutorial on this here: https://github.com/satijalab/seurat/discussions/4137#discussioncomment-407760

Also look here for additional details on subclustering: https://github.com/satijalab/seurat/discussions/4122

Hope this helps!