DESeq2 error in design~ code
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3.0 years ago

Hi everyone I need to run Deseq2, but when I try to run this part of command the following error message appears.

Error in DESeqDataSet(se, design = design, ignoreRank) : 
  design has a single variable, with all samples having the same value.
  use instead a design of '~ 1'. estimateSizeFactors, rlog and the VST can then be used
De plus : Warning message:
In DESeqDataSet(se, design = design, ignoreRank) :
  1 duplicate rownames were renamed by adding numbers 

I am new to R and DESeq2, Please anyone could hepl me to fix this error. Thank you very much

EnhancedVolcanoplot DEseq2 • 1.7k views
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Please, provide your code. The error says design has a single variable, with all samples having the same value.. How have you designed your study?

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