Hi everyone I need to run Deseq2, but when I try to run this part of command the following error message appears.
Error in DESeqDataSet(se, design = design, ignoreRank) : design has a single variable, with all samples having the same value. use instead a design of '~ 1'. estimateSizeFactors, rlog and the VST can then be used De plus : Warning message: In DESeqDataSet(se, design = design, ignoreRank) : 1 duplicate rownames were renamed by adding numbers
I am new to R and DESeq2, Please anyone could hepl me to fix this error. Thank you very much