Entering edit mode
2.5 years ago
chansik
▴
10
I've downloaded bam files from ENA, and tried samtools flagstat to confirm mapping.
But the result was like this. How do I interpret this?
Should I download fastq files?
Thanks
Thank you, GenoMax!
I downloaded fastq files (read1 and read2) and ran fastQC but the quality doesn't seem any better. So I further deleted 15 bp of 5'end is it okay??
original fastq: https://www.dropbox.com/s/h1p4z51d3jk14et/ERR2593198_1_fastqc.html?dl=0 Trimmed (ngsShrot): https://www.dropbox.com/s/9cdcndrbawo91im/trimmed_ERR2593198_1_fastqc.html?dl=0 15bp trimmed: https://www.dropbox.com/s/pcqslx57jt4064a/15bp_trimmed_ERR2593198_1_fastqc.html?dl=0
Best regards,
This looks like this is an RNAseq experiment. There should be no need to delete 15 bp at 5'-end since it is because of this observation. You should either just trim to remove adapters or directly align to the genome/transcriptome.
You can add images to biostars posts using these directions.
Thank you again. I really appreciate your answer.
I just trimmed 15 bp at 5'end and this works pretty well in further processing