I use regularly BLASTp analysis and I would like to improve my command line so that only resulting hits with 60% of similarity and 60 % of coverage appear in the output file.
For the coverage, I use the following option that works great:
However, does anyone know what is the option to filter reads over 60% of similarity using blast directly?
I usually do it manually using an awk command but I would rather use blast to filter the resutls directly if possible.
Thank you very much for your help and advice!