I have been trying to assemble a human genome that has been sequenced on Nanopore-PromethION, basecalled on the latest version of bonito, and am now trying to run an assembly using shasta. The basecalling had no issues, but now when I try to run shasta, I get this error message:
> Read rate: 2.46759e+09 bytes/s. > Found 12964000 lines in this file. > 2021-Oct-13 16:56:18.278195 A runtime error occurred in thread 75: > Read at offset 59230953264 does not begin with "@".
I have been trying to look up this 'read at offset' issue for fastq files but so far no luck. Also, I am not quite sure how to look up specifically which read it is referring to that 'does not begin with "@"'. Any help would be much appreciated!