calculation of gene coverage
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5 weeks ago

I am beginner in bioinformatics and working on NGS for my Ph.D. project. I have illumina and ONT reads of yeast. I would like to know the tool to combine or hybridize and after calculation how to calculate coverage of a specific gene of interest inserted in genomic DNA?

coverage tools gene • 160 views
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Entering edit mode
5 weeks ago
prasundutta87 ▴ 580

Check out Qualimap bamqc (http://qualimap.conesalab.org/doc_html/analysis.html#bamqc)

Give your gene coordinates of interest as a GFF or BED file in the Regions file option (-gff, OR --feature-file). Hope this helps.

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