Based on biojava, I personally made and am using something that works with blastN.
I was using the NR and NT DB of v4 locally. However, there was one problem after updating this v5.
I tried to read and xml the pasta file with version 2.11.0 of BLAST+. (This is for Excel parsing.)
It works well in the database of v4, but v5 doesn't give me the results. (0 bytes)
The following are the options I used.
[blastn, , -task, megablast, -db, db_loc -query, C:\Users\Cosmos\Desktop\report.fas, -num_threads, 4, -num_alignments, 10, -num_descriptions, 10, -out, C:\Users\Cosmos\Desktop\blast.xml, -outfmt, 5]
Is there anything I haven't checked?
I look forward to your advice.