Why only reverse fatsq file is used for mapping step in single cell RNA sequencing analysis?

Question

[nazaninhoseinkhan]

Dear all,

I am following a tutorial to do ScRNA-Seq analysis.

In the mapping step it has been advised to use the reverse strand, however I don't understand the logic behind this.

Can anyone explain to me why we should only use the reverse file for mapping?

Regards

Answer 1

[cpad0112]

R1 is bar codes+UMI

[nazaninhoseinkhan]

Thank you so much

So it means that both reverse and forward strands have been merged in R2?

[cpad0112]

There is no merging.