Entering edit mode
2.9 years ago
nazaninhoseinkhan
▴
530
Dear all,
I am following a tutorial to do ScRNA-Seq analysis.
In the mapping step it has been advised to use the reverse strand, however I don't understand the logic behind this.
Can anyone explain to me why we should only use the reverse file for mapping?
Regards
Thank you so much
So it means that both reverse and forward strands have been merged in R2?
There is no merging.