Why only reverse fatsq file is used for mapping step in single cell RNA sequencing analysis?
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2.9 years ago

Dear all,

I am following a tutorial to do ScRNA-Seq analysis.

In the mapping step it has been advised to use the reverse strand, however I don't understand the logic behind this.

Can anyone explain to me why we should only use the reverse file for mapping?

Regards

ScRNA-Seq • 1.0k views
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2.9 years ago

R1 is bar codes+UMI

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Thank you so much

So it means that both reverse and forward strands have been merged in R2?

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There is no merging.

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