Using TCGA RSEM data to calculate isoform expression

Question

[bnfrmtcs]

Hello everyone,

I have download the TCGA RNAseq RSEM data about isoform expression. I would like to check which one of the isoforms of my gene is the one expressed the most. Can I directly use the RSEM values to conclude that ? Or should I do some sort of normalization before the statistical analysis?

[wwu222]

Hello, I think you can compare between isoforms using TPM values. By the way, may I ask where did you download RSEM isoform expression data for TCGA samples? Thanks!