Entering edit mode
2.4 years ago
YANG
•
0
Hi,
I received some basecalled fast5 file from company, and want to convert it to fastq. I tried poretools, but it doesn't work.
I am currently using guppy to do so as following command:
guppy_basecaller --flowcell FLO-MIN106 --kit SQK-RAD004 -r -i <directly to fast5> -s guppy_out
Do I make it right? I think this might be another basecalling process which I don't need. All my files are about 5 GB, and the Init time is 1341 ms. I really hope that this command can help me obtain fastq files.
