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2.4 years ago
Info.shi
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30
Hi everyone! I have a query regarding the consensus sequence I used the below command to create a consensus sequence.
bcftools mpileup -f ref.fasta mapped.bam -d 80000 | bcftools call -c | vcfutils.pl vcf2fq > cons.fq
If I see a reference sequence it shows "GGGA" as I expected it should be "GGGC" in consensus but I get "GGGA" in the consensus sequence.
I checked the VCF file of that variant is not mentioned for the particular gene but I can't understand why it is like so.
Please kindly advise me!
Thank you!