RiboZero RNA Seq vs Poly A RNA seq

Question

[student12]

Dear Community,

I wanted to know whether there is a difference in the downstream analysis of RiboZero RNA Seq data and Poly A+ RNA seq data?

I generally follow the pipeline shown below, is there something different that I need to do for RiboZero RNA seq data?

PIPELINE: FastQC > Adapter trimming > Alignment > feature Count (Count Matrix) > Differential Expression Analysis

Thank you

Answer 1

[Carlo Yague]

In general, yes, you can use the same pipeline for differential expression analysis.