hi all, I am working on miRNA alignment using bowtie. But it seems to fail to map. Here are the commands:
bowtie-build -f miRNA.fa miRNA bowtie --norc -k 1 -v 0 /public/home/wschen_gibh/cpa-seq/ref/miRNA/miRNA -1 SRR16643593_1.NEW.trim.fq -2 SRR16643593_2.NEW.trim.fq -S test.sam
and here is the result:
and the output sam file:
Could anyone help? thanks a lot!