help with filtering sequences to make a phylogenetic tree

Question

[DJ_MB]

Hi everyone

I have a fasta with around 400 sequences, and I have to make a phylogenetic tree, before that I have to eliminate the duplicate sequences, I was thinking of doing it manually with an alignment and a distance matrix but due to the quantity the work is somewhat slow.

Does anyone have any method to do this more quickly?

Answer 1

[cfos4698]

A quick and dirty way would be to start building an ML tree with iqtree. The program detects identical sequences and outputs a file at the very beginning that only contains unique sequences.