how to visually compare BAM file differences
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2.4 years ago
tan ▴ 10

I am a Bioinformatics novice learning workflow of calling somatic mutation . I found actions related to BAM file are these :

sort, markdup ,reorder ,indel realignment,BQSR , I want to known the differences of them after I execute one step .

in fact ,I'm a little confused ,GATK 3 and GATK 4 are somewhat different, There are also many software similar to GATK, I don't know the necessity of each step, maybe there will be a better way to learn, thank you for all your help

mutation tumor GATK bam somatic • 487 views
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