I have downloaded TCGA data generated from TOIL RNA-seq pipeline. Basically, it is log2(x+0.001) transformed transcript-level expression values (FPKM) matrix. Link to dataset
I intend to analyze isoform switching using IsoformSwitchAnalyzeR which won't work with log2 transformed data or negative values.
So I need to revert the Log2 (FPKM+0.001) to FPKM to remove the negative values but I'm not sure how?
Will taking antilog using 2^(FPKM_log2T_value) work? If not, then is there any other way?
Your help will be much appreciated.