My annotation file from Prokka looks like this: Does prokka annotation has any information that can be used to get GO terms using any tool? I see some of the genes have "Similar to AA Sequence: UniProtKB". Can I use this to get GO terms from Uniprot? Does the gene ID used in the first column follow any format that can be used anywhere to get GO terms? Uniprot does not recognize it. What about Prodigal number? Is it linked to the gene annotation and GO term information? Thank you.
Thank you for the reply. Probably, the only option I have is to pick UniprotKB ID from "Similar to AA Sequence: UniprotKB" and then get GO terms from Uniprot. I also have the sequence of each gene (and amino acid sequence) in a FASTA format. It has a couple of hundred genes. Can the FASTA file be used to get GO Terms?