Difference between "--solostrand" and "--readstrand" commands in STARsolo
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5 months ago
Aaron ▴ 10

Would someone be able to explain the difference between the "--solostrand" and "--readstrand" commands in STARsolo? What would it look like if I set each "Unstranded" vs e.g. "Forward"? I'm reading documentation for it (https://physiology.med.cornell.edu/faculty/skrabanek/lab/angsd/lecture_notes/STARmanual.pdf) but I'm not exactly clear about it. (This is my first time analyzing RNA-seq data though.)

STARsolo • 218 views
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