Scaling and Centering After VST

Question

[weimichelles]

Hello, after VST transformation of raw RNA-seq counts, is it necessary to scale (divide the expression value of each gene by the standard deviation of the expression of that gene) and center (subtract the mean expression value of the gene) the data? Sorry if this is a basic question, and thank you so much!

[ATpoint]

Depends on the analysis. What’s the analysis goal?

[weimichelles]

I'm trying to do LASSO regression, where the expression value of each gene is an independent variable.