Hi, I am super strugling with Agilent two colour microarray. I am moving forward, but super slowly. Now I am in the possession of a decent script where i have to upload Spottype file. All the files for this array I finally found but when i upload spottype file i get an error.
So what I see is below.
data=read.maimages(files = target, source ="agilent", green.only = F, columns = list(G="gMedianSignal",Gb="gBGMedianSignal",R="rMedianSignal",Rb="rBGMedianSignal"),
annotation = c("ProbeName","SystematicName") )
plotMD(object = data) spottypes=readSpotTypes(file = "Spots3.txt", check.names=FALSE)
Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec, : line 32133 did not have 20 elements
I even tried to delete this line but the error cames up all the time. Can someone please help me? Maybe I am uploading wrong file time as this Spottype file?