My aim is to carry out the following:
- Homology analysis of consensus sequences (nanopore platform, human) to TCR sequence reference databases and then assign the V, D, J, and C to individual sequences.
Next, obtain the following table:
TRAV TRJ CDR3 Reads %Reads
TRAV25 TRJ36 CPRKSQTGAANLFF 543 0.44
TRAV12-1 TRJ11 CVVNEGYSTLF 414 0.33
What is the best approach and what are the best tools?
Thanks in advance