I was trying to run nf-core/rnaseq test for the 1st time. It stops half-way through with the following error after rounds of trials:
Error executing process > 'NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT' Caused by: Process `NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT` terminated with an error exit status (1) Command executed: bbsplit.sh \ -Xmx6g \ ref_primary=genome_gfp.fasta \ ref_sarscov2=GCA_009858895.3_ASM985889v3_genomic.200409.fna ref_human=chr22_23800000-23980000.fa \ path=bbsplit \ threads=2 \ build=1 cat <<-END_VERSIONS > versions.yml "NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:BBMAP_BBSPLIT": bbmap: $(bbversion.sh 2>&1) END_VERSIONS Command exit status: 1 Command output: Error occurred during initialization of VM Could not reserve enough space for 6291456KB object heap
I don't know why it's like this and how to solve this issue so that I can proceed to analysis of my own datasets. I am running the workflow on HPC with memory of 50 G. Any response is greatly appreciated!