Hi guys how do you answer this:
You have first performed mutation calling from WGS in your tumor sample using blood as the normal control. Now looking at the RNAseq data from the same tumor sample, you notice that a heterozygous mutation in your gene of interest does not appear in the RNAseq reads even though there is plenty of coverage at this locus. Why might this be the case?
Hi Kevin Thanks you are always helpful. Please don't call my tutors they are crazy and overwhelmed us with too many questions for a short time and tomorrow morning is the deadline hahahha