Which method works best for analysing ONE sample of scRNA-seq data?
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4 months ago
solarchan7 • 0

Hello, I currently have a single-cell RNA-seq (scRNA-seq) data of a single person (sample) and i want to perform DE analysis. However, when I run the DESeq() in the DESeq2 package, it shows an error about only one sample is not allowed. What are some alternative to this? Thank you in adcance.

analysis RNA gene r DNA • 264 views
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What do you want to compare?

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4 months ago
fracarb8 ▴ 760

DESEq2 is design to analyse bulk RNA-Seq. For single-cell have a look at seurat, monocle3 or scanpy. I am sure there are more packages available, but these are the one I used and known.