I used Seurat to do clustering, then singleR to do cell annotation. In this annotation, I got multiple cell types such as B cells and T cells and Epithelial cells. I'm only interested in Epithelial cell, so I subset the Seurat object to only keep Epithelial cell. Then I redo clustering on Epithelial cell cluster. Here is the new clusters Seurat generated:
I want to assign cell names to each cluster, but I'm not sure how to do this on the further clustered data (I don't think I can use singleR again, because if SingleR can annotate these clusters, it should have done that at the first time I used it.)
I used seurat to calculate the gene expression in each cluster and hoping to get cell type based on the gene expression information. The gene expression table looks like this:
So my question is, does anybody know how to do cell annotation on these sub clusters? Is there any reference database that I can use to find cell names based on the gene expressions?
Thanks so much! Leran