How to specify the depth of sequencing in ion torrent?
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3 months ago
shersky • 0

I have never used ion torrent machine before, but in Illumina there is a calculator to specify the coverage before sequencing. Is there for ion torrent a similar thing, or in other words, can it be controlled in ion torrent sequencing ?

sequencing • 308 views
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3 months ago

The coverage of a sequencing run is the size of the genome divided by the number of total bases sequenced.

When an instrument produces identical read lengths then the formula is

 coverage = number_of_reads * lenght_of_each_read / genome_size


The genome size is the expected length of the DNA that gets fragmented.