First, I feel embarrassed to ask this seemingly fundamental question for all of you and I am very unfamiliar with FASTQ/BAM handling..... Recently, we are trying to design a probe for RNA scope (or maybe possibly antibody as well in the future) that we found in the scRNA sequencing study recently. Is there anyway that we extract the sequence of the gene (probably partial or intron-region genes) which was recognized as our gene of interest?
Thanks a tone in advance guys!!