RNAseq data with no biological replicates

Question

[dew]

How to deal with RNAseq data without biological replicates? Are the tools currently only edgeR(bcv) and Gfold tools?

Thanks a lot!

[Princy]

Hi, If you have no replicate for your samples, then you can also use NOISeq package. The Pairwise Differential Expression Analysis (Without Replicates) functionality offers a strategy for analyzing RNA-seq datasets that do not have replicates. It is based on the software package NOISeq, which belongs to the Bioconductor project. NOISeq is a novel nonparametric approach for the identification for differentially expressed genes from RNA-Seq count data.