Hello, I used MIXCR on standard Single Cell data and found some common clonotypes among my multiple conditions. I would like to know if it would be possible to track clonotypes with the TCR Sequence ? If yes, how ? I imagined I will have to search the TCR Sequence in the raw fastq file to identify to which cell it belongs with UMI Tools (I have fastq reads for each clonotype thanks to MIXCR) but I suppose there will be easier way to do that. The ultimate goal is to subset the clonotypes among my cells in Seurat and compare their expression between the different conditions.
Someone already tested something like that ?