I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help

Question

[mibtisamnasar]

I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help in this.

[swbarnes2]

It's not a pushbutton thing you are asking. Have you googled a variant calling tutorial?

Answer 1

[abedkurdi10]

You can use velvet or spades for reads assembly, then use blast on the obtained FASTA to know to which bacteria your samples resemble.