Entering edit mode
3.6 years ago
Shinster007
▴
10
Hello,
I have a general question. I am analyzing some mouse and human scATAC Seq data of the same tissue/cell types and would like to do some cross-species analyses. I am interested in looking at conserved peaks between my two datasets, something similar to what is done in this paper here (figure 3 and methods "Identification of conserved peaks between mouse and human":
https://www.cell.com/cell-reports/pdf/S2211-1247(21)01473-X.pdf
This would consist of clustering, peak calling, etc. of my two datasets independently, extracting the peak matrix from each, "humanizing" the mouse peak set with liftOver and then looking for overlaps. I'm just wondering how valid this approach is?
